Our real-time PCR outcomes confirmed null Cq values in majority of the adverse high precision machining management samples. When high Cq values (e.g. 39 and above) have been noticed with these adverse management samples, it was also noticed with NTC samples added to the identical PCR run. The high Cq values present in adverse management and NTC can be attributed to very small quantity of goal DNA or amplicons (e.g., 1 copy per PCR) find yourself being in the PCR master mix when the reagents have been ready.
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Our real-time PCR outcomes confirmed null Cq values in majority of the adverse high precision machining management samples. When high Cq values (e.g. 39 and above) have been noticed with these adverse management samples, it was also noticed with NTC samples added to the identical PCR run. The high Cq values present in adverse management and NTC can be attributed to very small quantity of goal DNA or amplicons (e.g., 1 copy per PCR) find yourself being in the PCR master mix when the reagents have been ready.
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